Analysis of oxidized phosphatidylcholines using structure specific survey or focused multiple reaction monitoring with reverse phase LC/MS/MS

摘要

Oxidized phospholipids (oxPLs) and their decomposition products are occurred under many different types of oxidative stress and closely related to the various diseases. Oxidized low-density lipoprotein (oxLDL), containing large amounts of oxPLs, is known to cause atherosclerosis. Thus, analysis of oxPLs is very important to understand these physiological phenomena. For analysis of oxPLs, many methods have been applied. But, oxPLs have short longevities in vivo and a lot of isomers. In addition, micro heterogeneities can occur within oxPLs or non-oxPLs. So, analysis of oxPLs is very difficult. In this study we firstly applied structure-related survey for oxidized phosphatidylcholines (oxPCs) by using fragments for oxPCs. Then we tested focused multiple reaction monitoring (MRM) by using theoretical fragment pattern of oxPCs.