LC-MS / MS method of monitoring multiple reactions to detect therapeutic antibodies in animal samples using frame-changing peptides

摘要

A method of detecting human or humanized antibodies comprising the steps of: (a) treating a biological sample comprising a human or humanized antibody with a digestive enzyme to form a digested antibody sample, wherein the biological sample is serum, plasma , tissue or cells from an animal that has been treated with a human or humanized antibody; and (b) analyzing the antibody specimen digested by mass spectrometry to detect one or more peptides present in human antibodies and not present in animal antibodies, where the peptides comprise one or more sequences selected from SEQ ID NO: 1- 8, ** Formula ** where the digestive enzyme is trypsin.