Development and validation of LC-MS/MS method with multiple reactions monitoring mode for quantification of vanillin and syringaldehyde in plum brandies

摘要

An ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS) method with multiple reactions monitoring mode (MRM) has been developed and validated for quantification of vanillin and syringaldehyde in plum brandy. The method showed good linearity (0.05 to 10 mgL?1) and low limits of detection and quantification (LOD and LOQ were 11.6 μgL?1 and 38.2 μgL?1 for vanillin, and 12.7 μgL?1 and 42.0 μgL?1 for syringaldehyde, respectively). The overall intra-day and inter-day variations were less than 4.21%, and the overall recovery over 93.0%. The correlation coefficients (R2) of the calibration curves were higher than 0.9999. In order to evaluate if the method is suitable for use as a routine analytical tool, in 31 Serbian plum brandy samples vanillin and syringaldehide were determined. [Projekat Ministarstva nauke Republike Srbije, br. 172053]