Synthesis and Characterization of Bifunctional Nano Gene Vector

摘要

This paper describes the synthesis of Fe_3O_4/Poly[3-(trimethylamino)ethyl methacrylate iodine] (PTMAI)/CdTe bifunctional nano gene vector for targeting DNA delivery. In this approach, Poly[2-(dimethylamino)ethyl methacrylate] (PDMA) was grafted on the Fe_3O_4 nanoparticles by surface-initiated transfer radical polymerization (ATRP). The ternary amine groups of PDMA can be alkylated by the iodomethane to obtain PTMAI. The quaternary amine groups of PTMAI on the nanopaticles can adsorb the Plasmid DNA and CdTe nanocrystals with carboxyl group by self-assembly approach to form the nanobiomaterial. CdTe nanoparticles could label Plasmid DNA and magnetic nanoparticles could target DNA delivery. The use of the water-solubility magnetic Fe_3O_4/PTMAI nanocomposite particles for the controlled release and directed DNA delivery in biological systems is important.