PROCESS FOR DIRECT, TARGETED AND REPRODUCIBLE GENETIC MANIPULATION, BIFUNCTIONAL VECTORS, PRODUCTION MICROORGANISMS OF BACILLUS STRAIN TRANSFORMED BY BIFUNCTIONAL VECTOR, INSECTICIDAL AGENT AND USE OF BIFUNCTIONAL VECTORS

摘要

The present invention describes a process which makes it possible to carry out direct and targeted genetic manipulation of Bacillus thuringiensis and the closely related B. cereus using recombinant DNA technology. The present invention also relates to the construction of plasmids and shuttle vectors and to the B. thuringiensis strains transformed therewith. Likewise described is a process for the direct cloning, expression and identification of genes in B. thuringiensis and the closely related B. cereus. IMAGE