Optimization of Extraction of Flavonoids from Pteris multifida by Response Surface Methodology

摘要

The extraction of flavonoids from Pteris multifida was optimized to maximize flavonoid yield in this study. A central composite design of response surface methodology involving time, temperature, ratio of liquid to solid and ethanol concentration was used, and second-order model for flavonoid yield was employed to generate the response surface. The optimum condition for flavonoid extraction was determined as follows: temperature 65.6 °C, ethanol concentration 58.1 %, ratio of liquid to solid 20, time 90 min. The predicted yield at the optimum condition was 2.70 %. Experimental verification gave values of 2.66 %.