Ara h 3, a major peanut allergen, has not been fully researched, partially because its purification is quite difficult. In this study, anion exchange column chromatography was employed to purify Ara h 3 and concentrated. Relatively highly purified Ara h 3 was obtained by using appropriate protein purifier, suitably- sized anion exchange column and applicable elution conditions. This approach was characterized by several advantages, such as less steps and less time consuming. The established purification method with high efficiency and convenience would play an important role in Ara h 3 purification and further structural and functional study.
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机译:ARA H 3是一个主要的花生过敏原,尚未完全研究,部分是因为它的纯化非常困难。在该研究中,使用阴离子交换柱色谱法纯化ARA H 3并浓缩。通过使用适当的蛋白质净化器,适用的阴离子交换柱和适用的洗脱条件,获得相对高度纯化的ARA H 3。这种方法的特征在于几个优点,例如较少的步骤和较少的耗时。具有高效率和便利性的建立的纯化方法将在ARA H 3净化和进一步的结构和功能研究中发挥重要作用。
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