FUSION OF SURVIVIN AND GM-CSF, CODING DNA, RECOMBINANT EXPRESSION VECTOR, ANTI-TUMOR VACCINE, AND APPLICATION THEREOF

摘要

A fusion of survivin and GM-CSF, coding DNA, a recombinant expression vector, an anti-tumor vaccine, and an application thereof. The fusion of survivin and GM-CSF includes survivin and GM-CSF that are operably linked. The DNA encoding the fusion of survivin and GM-CSF includes a survivin coding element and a GM-CSF coding element that are operably linked, wherein the survivin coding element encodes the amino acid sequence of survivin, and the GM-CSF coding element encodes the amino acid sequence of GM-CSF. The fusion effectively improves the immunogenicity of a survivin protein, and the constructed recombinant BCG vaccine achieves the effects of both a BCG vaccine vector and a therapeutic drug, and achieves the synergistic effect of an antigen, an adjuvant, and a vaccine vector, so that the immunogenicity of a tumor vaccine is more effective and lasting.