Induction of Apoptosis of Human Colon Cancer Cells by siRNA Recombinant Expression Vector Targeting Survivin Gene

摘要

This study constructed siRNA recombinant expression vector targeting survivin gene and observe the apoptosis induction effect of it in human colon cancer cells,siRNA recombinant expres-sion vector targeting survivin gene was constructed and transfected into human colon cancer cells.The effect of siRNA recombinant expression vector was detected by RT-PCR,Western blot,MTT re-duction assay and flow cytometry.It was confirmed by restriction endonuclease and sequence analy-sis that siRNA recombinant expression vector targeting survivin gene was constructed successfully.Inhibition rate of survivin siRNA at mRNA and protein levels was 36.33% and 44.65% respectively.Growth of cancer cells was inhibited and the apoptosis rate was (17.24±2.13)%.The siRNA recom-binant expression vector targeting survivin gene has been constructed successfully.It not only can in-hibit the expression of survivin gene,but also can induce apoptosis in human colon cancer cells re-markably.