The present invention relates to a composition for inhibiting biofilm formation of pathogenic microorganisms containing centipede oil as an active ingredient, wherein the composition containing centipede oil as an active ingredient contains a biofilm and As it was confirmed that it inhibits mycelial growth, and exhibits excellent anti-biofilm activity against multiple biofilms generated by Candida albicans and Staphylococcus aureus, the composition containing the centipede oil as an active ingredient is an effective biofilm-inhibiting composition and pathogenicity It can be provided as a therapeutic agent for infectious diseases caused by the biofilm of microorganisms.
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