Methods to specifically stimulate the survival and expansion of genetically modified immune cells

摘要

The present invention relates to a vector encoding a wild-type or truncation form of erythropoietin receptor (EpoR) to promote T cell survival and proliferation. Specifically, it has an anti-CD19-41 BB-003ζ chimeric antigen receptor (CAR) that has a greater ex vivo expansion than CAR-T cells and exhibits significantly higher anti-leukemia activity in vivo. The bicistronic vector to be expressed is illustrated. It also describes a method of generating cells expressing the vector and the use of these cells to kill CD19+ tumor cells.