Method for the preparation of monoclonal antibody derived from immunoreactive trypsin

摘要

A method for the preparation of monoclonal antibody (MAb) derived from immunoreactive tyrypsin (IRT) including the steps of:(i) obtaining purified human IRT or extract containing same; and(ii) forming antibody to said derivative or said extract by cloning antibody producing cells from an animal having been administered thereto said derivative of said extract.;There is also included a method of detection of IRT in a fluid sample using the aforementioned monoclonal antibodies wherein a fluid sample suspected of containing IRT is contacted with the aforementioned monoclonal antibodies and the resulting complex subjected to a detection step which may be RIA or EIA. There is also provided a method of diagnosis of cystic fibrosis depending upon the result of the protection step having regard to the concentration levels of IRT in the fluid sample. There is also provided a method of diagnosis of pancreatic damage wherein the presence of IRT in the fluid sample is analysed to determine the concentration levels.