Brain & Nerve Research Laboratory, Department of Neurological Surgery, Suzhou Medical College, Jiangsu;
Brain & Nerve Research Laboratory, Department of Neurological Surgery, Suzhou Medical College, Jiangsummunoconjugates are antibody-drug hybrid molecules which combine the exquisite selectivity or monoclonal antibodies with the potent toxicity of anticancer agents. A monoclonal antibody SZ39 against human brain gliomas was used as a drug carrier. Adriamycin (ADR) was bound covalently to SZ39 to form a SZ39-ADR conjugate. The cytotoxic activity of the SZ39-ADR conjugate was tested in vitro and demonstrated potent and specific killing of cells derived from a human malignant glioma. 50% inhibitory concentration (IC50) for SZ39-ADR to "target" cells was 8.14×10-9 M. An index of specificity between "target" and "non-target" cells was calculated to be 88-fold. These data suggest that the SZ39-ADR may use as a potent and cell type-specific agent and is a likely candidate for the targeting chemotherapy of malignant gliotnas.;
conjugate; covalently; potent; selectivity; monoclonal; likely; glioma; anticancer; cytotoxic; targeting;