首页> 外国专利> AN EFFICIENT METHOD FOR REGENERATING COTTON FROM CULTURED CELLS

AN EFFICIENT METHOD FOR REGENERATING COTTON FROM CULTURED CELLS

机译:从培养细胞再生棉的有效方法

摘要

Method for the production of proembryonic cotton cell biomass in liquid suspensions, comprising the following steps: (a) induction of cotton callus formation at approx. 20 DEG to 40 DEG C, by placing cotton plant tissue on a medium which is suitable for callus induction, followed by further cultivation steps for preventing discoloration; (b) suspension of up to 40 mg/ml of the callus in a suitable liquid medium which induces proembryonic cell biomass and which contains a relatively low concentration of at least one auxin until lumpy aggregations of proembryonic cell biomass are formed which multiply rapidly; and (c) transfer of the rapidly multiplying lumpy aggregations of the proembryonic cell biomass to a liquid nutrient medium in which smaller, more finely-dispersed proembryonic cell biomass can be formed, where said medium contains a relatively high concentration of at least one auxin. The proembryonic cell biomass is capable of developing mature embryos, small plantlets and, finally, mature plants. IMAGE
机译:用于在液体悬浮液中生产前胚棉细胞生物质的方法,包括以下步骤:(a)在约200℃诱导棉花愈伤组织的形成。通过将棉花植物组织放在适于愈伤组织诱导的培养基上,在20℃至40℃下进行,随后进行进一步的培养步骤以防止变色; (b)将高达40mg / ml的愈伤组织悬浮在合适的液体培养基中,所述液体培养基诱导前胚细胞生物质并且包含相对低浓度的至少一种生长素,直到形成前胚细胞生物质的团块状聚集并迅速繁殖; (c)将前胚细胞生物质的快速繁殖的块状聚集体转移到液体营养培养基中,在其中可以形成更小,更细分散的前胚细胞生物质,其中所述培养基包含相对高浓度的至少一种植物生长素。前胚细胞生物质能够发育成熟的胚,小植株,最后发育成熟的植物。 <图像>

著录项

  • 公开/公告号ZA888599B

    专利类型

  • 公开/公告日1990-07-25

    原文格式PDF

  • 申请/专利权人 CIBA-GEIGY A.G.;

    申请/专利号ZA19880008599

  • 发明设计人 JOHN FINER;JOHN FINER;

    申请日1988-11-17

  • 分类号C12M;C12N;

  • 国家 ZA

  • 入库时间 2022-08-22 06:17:37

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