Molecular diagnostic method for the detection of Brucella abortus and differentiation of the vaccine strain b19.

摘要

MOLECULAR DIAGNOSTIC METHODS FOR SCREENING AND CVABRUCELLA ABORTUS Differentiating VACCINE STRAIN B19. They DESCRIBED A NUMBER OF MOLECULAR DIAGNOSTIC METHODS ALLOWING A differentiation between ESTIRPE VACCINE CVABRUCELLA ABORTUS B19 AND ALL OTHER STRAINS OF THE GENUS CVABRUCELLA. IN A CASE APPLIED GENOME amplification, oligonucleotide primers using as DERIVED FROM region containing the genes encoding utilization pathway erythritol. Another group of methods is based on genomic DNA hybridization using as PROBES Oligonucleotides CVAERI that region. IN THIS CASE CAN BE APPLIED ACCORDING technique called "Southern blot" or alternatively by THE PROCEDURE "DOT BLOT". FINALLY THESE METHODS FOR IDENTIFYING GERMS OF THE GENUS CVABRUCELLA apply in any biological sample. NEW PROCEDURES ARE ON TRADITIONAL METHODS Advantageous, immunological or cultured, providing greater specificity and speed.