ELECTROBIOCHEMICAL ANALYTICAL METHOD AND ELECTRODES

摘要

An analytical method for determining the presence or concentration of an analyte in a liquid medium comprising the steps of: selecting an electrobiochemical system, the electrobiochemical system comprising a test electrode which has chemisorbed, sulfur-containing moieties; selecting a redox enzyme, the redox enzyme selectively converting the analyte to a product; binding the redox enzyme to the test electrode; selecting an electron mediator material which transfers electrons to and from the redox enzyme and test electrode; introducing a liquid medium with an analyte to the electrobiochemical system; applying a constant or alternating electrical potential to the test electrode so that electrons pass between the redox enzyme and the test electrode allowing the redox enzyme to catalyze the reaction of the analyte; and measuring the concentration of the analyte.