Methylmercury is widely known to be a toxic substance in the human, especially a nervous system. However, it is difficult to accurately measure the amount of methylmercury in blood, and the form of methylmercury is variously presented. The purpose of study was to compare the total mercury and methylmercury measurements techniques and detection levels between analytical institutions in two countries using the same elderly human blood samples. Total mercury using gold amalgamation direct mercury analysis method (both) and methylmercury using the dithizone extraction and gas chromatography-electron capture detector (GC-ECD) method (N Lab in Japan) and the cold vapor atomic fluorescence spectrophotometer (CVAFS) method (D Lab in Korea) were measured in 47 subjects who agreed to participate in this study. Total mercury concentrations in both analytical laboratories were observed at similar levels (9.4 versus 9.5 ug/kg, p=0.898) and the distribution was highly correlated. However, the concentration of methylmercury showed some difference between two laboratories (9.1 versus 8.6 ug/kg, p<0.001). Due to different recovery rates by different analytical methods, it is assumed that the methyl/total mercury ratio in N lab in Japan was higher than D lab in Korea (96.8 versus 90.4%, p<0.001). The GC-ECD was more sensitive method than CVAFS in methylmercury analytic techniques.
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机译：众所周知，甲基汞是人类尤其是神经系统的有毒物质。然而，难以准确地测量血液中甲基汞的量，并且存在多种形式的甲基汞。研究的目的是比较使用相同的老年人血液样本的两个国家的分析机构之间的总汞和甲基汞测量技术和检测水平。使用汞齐直接汞法的总汞含量（两种）和使用双硫zone萃取和气相色谱-电子捕获检测器法（日本N Lab）和冷蒸气原子荧光分光光度计（CVAFS）法（D）的甲基汞在韩国实验室）对同意参加这项研究的47位受试者进行了测量。在两个分析实验室中观察到的总汞浓度处于相似的水平（9.4对9.5 ug / kg，p = 0.898），并且分布高度相关。但是，两个实验室之间的甲基汞浓度存在一些差异（9.1对8.6 ug / kg，p <0.001）。由于不同分析方法的回收率不同，因此可以推测，日本N实验室的甲基/总汞比高于韩国D实验室（96.8对90.4％，p <0.001）。在甲基汞分析技术中，GC-ECD比CVAFS更灵敏。
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