APG 12 GENE ESSENTIAL TO AUTOFUZZY, ITS DETECTION, MANUFACTURE OF RECOMBINANT PROTEIN BASED ON ITS GENE SEQUENCE, MANUFACTURE OF ANTIBODY AGAINST THE SAME AND DETECTION OF APG 12 PROTEIN USING ITS ANTIBODY

摘要

PROBLEM TO BE SOLVED: To obtain a new APG 12 gene which consists of APG 12 gene containing a specific base sequence and essential to autofuzzy and is useful in elucidating or the like at molecular level the mechanism of the phenomenon that autologous components are decomposed when the nutrient source from the outside is exhausted. ;SOLUTION: This gene is a new APG 12 gene represented by formula I-III and essential to autofuzzy and is useful in elucidating at molecular level the mechanism of autofuzzy phenomenon that cells decompose a part of autologous constitutive components to reutilize as nutrient sources when the nutrition sources from the outside are exhausted and diagnosing or treating diseases, e.g. Alzheimer disease, pathological protein accumulation disease or the like, caused by proteins which should be decomposed and are accumulated due to the malfunction in the autofuzzy mechanism. This APG 12 gene is obtained by amplifying and detecting the gene of bud yeast, human being or mouse by means of PCR method with the combination of complementary oligonucleotides.;COPYRIGHT: (C)2000,JPO