Dengue-1 Virus Envelope Glycoprotein Gene Expressed in Recombinant BaculovirusElicits Virus-Neutralizing Antibody in Mice and Protects them from Virus Challenge. (Reannouncement with New Availability Information)

摘要

In an effort explore the feasibility of a baculovirus recombinant dengue-1 (DEN1) virus vaccine, a portion of the envelope (E) glycoprotein gene from DEN 1 virus was cloned and expressed in baculovirus (Autographa californica nuclear polyhedrosis virus, AcNPV). The recombinant construct contains 107 nucleotides from the 3' terminus of the M gene which encodes a hydrophobic signal peptide and extends through the first 1245 nucleotides of E, terminating 243 nucleotides before the 3' terminus of E. When the recombinant was grown in Spodoptera frugiperdal cells, about 1 mg of E antigen was made per 1