DNA FRAGMENT HC 250 PREPARED BY CHEMICAL SYNTHESIS, RECOMBINANT POLYPEPTIDE HC 250 OF MOLECULAR MASS 120-130 KDA SHOWING CAPABILITY TO BIND ANTIBODIES TO GENE-ENCODING PROTEIN NS3 OF HEPATITIS C VIRUS PREPARED BY CULTIVATION OF THE STRAIN OF BACTERIUM ESCHERICHIA COLI AND STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF RECOMBINANT POLYPEPTIDE HC 250 SHOWING CAPABILITY TO BIND ANTIBODIES TO GENE-ENCODING PROTEIN NS3 OF HEPATITIS C VIRUS

摘要

FIELD: molecular biology, biotechnology. SUBSTANCE: invention relates to preparing DNA fragment HC 250 that determines synthesis of polypeptide showing properties of human hepatitis C virus protein NS3. Polypeptide is prepared using the strain of bacterium Escherichia coli transformed with plasmid carrying DNA fragment HC 250. This polypeptide is able to bind antibodies to gene-encoding protein NS3 of hepatitis C virus. Invention is used in immunodiagnosis of human hepatitis C virus. EFFECT: improved method of preparing. 3 cl