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CONTEMPORARY DIFFERENTIAL DIAGNOSTIC METHOD KIT AND MANUFACTURING METHOD OF HEMORRHAGIC FEVER WITH RENAL SYNDROME TSUTSUGAMUSHI DISEASE AND LEPTOSPIROSIS USING IMMUNOBLOT
CONTEMPORARY DIFFERENTIAL DIAGNOSTIC METHOD KIT AND MANUFACTURING METHOD OF HEMORRHAGIC FEVER WITH RENAL SYNDROME TSUTSUGAMUSHI DISEASE AND LEPTOSPIROSIS USING IMMUNOBLOT
The present invention relates to a simultaneous differential diagnosis method of nephrotic bleeding fever, Tsutsugamus disease, leptospirosis using immunoblot, and more particularly, to blot the antigens of nephrotic bleeding fever, Tsutsugamus disease and leptospirosis at a predetermined position on the nitrocellulose membrane, respectively. step; Reacting the sample and the control serum with the nitrocellulose membrane blotted with the antigens, respectively; Detecting the antigen-antibody reactants produced by the reaction using alkaline phosphatase-labeled goat anti human immunoglobulin antibody and BCIP / NBT substrate solution, respectively; And it relates to a simultaneous differential diagnosis of nephrotic bleeding fever, Tsutsugamus disease, leptospirosis comprising the step of comparing the detection results in the sample and control serum.;The present invention also relates to a diagnostic kit comprising an antigen-blotting nitrocellulose membrane obtained by blotting antigens of nephrotic bleeding fever, Tsutsugamus disease and leptospirosis, respectively, on a nitrocellulose membrane.;According to the present invention, it is possible to easily diagnose nephrotic bleeding fever, Tsutsuma's disease, and leptospirosis at the same time without a skilled inspector and special equipment.
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