首页> 外国专利> CONTEMPORARY DIFFERENTIAL DIAGNOSTIC METHOD KIT AND MANUFACTURING METHOD OF HEMORRHAGIC FEVER WITH RENAL SYNDROME TSUTSUGAMUSHI DISEASE AND LEPTOSPIROSIS USING IMMUNOBLOT

CONTEMPORARY DIFFERENTIAL DIAGNOSTIC METHOD KIT AND MANUFACTURING METHOD OF HEMORRHAGIC FEVER WITH RENAL SYNDROME TSUTSUGAMUSHI DISEASE AND LEPTOSPIROSIS USING IMMUNOBLOT

机译:免疫斑点法鉴别诊断肾病综合症和汗液性出血热的当代鉴别诊断试剂盒和制造方法

摘要

The present invention relates to a simultaneous differential diagnosis method of nephrotic bleeding fever, Tsutsugamus disease, leptospirosis using immunoblot, and more particularly, to blot the antigens of nephrotic bleeding fever, Tsutsugamus disease and leptospirosis at a predetermined position on the nitrocellulose membrane, respectively. step; Reacting the sample and the control serum with the nitrocellulose membrane blotted with the antigens, respectively; Detecting the antigen-antibody reactants produced by the reaction using alkaline phosphatase-labeled goat anti human immunoglobulin antibody and BCIP / NBT substrate solution, respectively; And it relates to a simultaneous differential diagnosis of nephrotic bleeding fever, Tsutsugamus disease, leptospirosis comprising the step of comparing the detection results in the sample and control serum.;The present invention also relates to a diagnostic kit comprising an antigen-blotting nitrocellulose membrane obtained by blotting antigens of nephrotic bleeding fever, Tsutsugamus disease and leptospirosis, respectively, on a nitrocellulose membrane.;According to the present invention, it is possible to easily diagnose nephrotic bleeding fever, Tsutsuma's disease, and leptospirosis at the same time without a skilled inspector and special equipment.
机译:本发明涉及利用免疫印迹同时诊断肾病性出血热,tsutsugamus病,钩端螺旋体病的方法,更具体地说,涉及在硝酸纤维素膜上的预定位置分别印迹肾病性出血热,tsutsugamus病和钩端螺旋体的抗原。步;使样品和对照血清分别与被抗原印迹的硝酸纤维素膜反应;分别使用碱性磷酸酶标记的山羊抗人免疫球蛋白抗体和BCIP / NBT底物溶液检测反应产生的抗原抗体反应物;并且涉及同时鉴别诊断肾病性出血热,tsutsugamus疾病,钩端螺旋体病的步骤,包括比较样品和对照血清中的检测结果的步骤。通过在硝酸纤维素膜上分别印迹肾病性出血热,tsutsugamus病和钩端螺旋体病的抗原。根据本发明,无需熟练的检查员就可以同时容易地诊断肾病性出血热,tsutsuma氏病和钩端螺旋体病。和特殊设备。

著录项

  • 公开/公告号KR100234876B1

    专利类型

  • 公开/公告日1999-12-15

    原文格式PDF

  • 申请/专利权人 BORYUNG PHARMACEUTICAL CO. LTD;

    申请/专利号KR19970015237

  • 发明设计人 이평우;정한선;

    申请日1997-04-24

  • 分类号G01N33/53;

  • 国家 KR

  • 入库时间 2022-08-22 01:46:20

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