RESOLVASE-CATALYSED, SEQUENCE-SPECIFIC DNA-RECOMBINATION IN EUKARYOTIC CELLS

摘要

The invention relates to a method for the sequence-specific recombination of DNA in eukaryotic cells, comprising the following steps: introducing a first DNA sequence which contains a res sequence into a cell, introducing a copy of the first DNA sequence and performing the sequence-specific recombination using the influence of a resolvase or its derivative. The invention also relates to a method for testing topoisomerase inhibitors in a eukaryotic cell, comprising the following steps: introducing a first DNA sequence according to SEQ ID NO:1 into a cell, introducing a copy of the first DNA sequence according to SEQ ID NO:1 in direct orientation, introducing a wild-type resolvase and adding the topoisomerase inhibitors which are to be tested.