METHOD FOR DETECTING UNKNOWN OR KNOWN NUCLEIC ACID MUTATION USING NUCLEIC ACID MELT CURVE AND NUCLEIC ACID DISSOCIATION CURVE, AND EXHIBITION METHOD THEREOF

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting an unknown and known nucleic acid mutation rapidly, easily and at a low cost, and an exhibition method thereof.;SOLUTION: Problems are solved by a method for detecting a nucleic acid mutation through a difference and a feature of a temperature (Tm value) in which a double-stranded nucleic acid or an amplification product thereof is heated to melt into a single-stranded nucleic acid. The unknown and known nucleic acid mutation can be detected rapidly, easily and at a low cost by comparing and collating a Tm value, a Tm pattern and a change thereof of a sample with an obtained existing Tm value, a Tm pattern and a change thereof accumulated as a data base.;COPYRIGHT: (C)2003,JPO