首页> 外文期刊>高等学校化学研究(英文版) >Fluorescence Microscopic Image Analysis of Nucleic Acids Based on The Capillary Flow Directed Assembly Ring of Neutral Red-nucleic Acid Supramolecular Complexes
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Fluorescence Microscopic Image Analysis of Nucleic Acids Based on The Capillary Flow Directed Assembly Ring of Neutral Red-nucleic Acid Supramolecular Complexes

机译:基于中性红核酸超分子配合物的毛细管流动定向组装环的核酸荧光显微图像分析

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摘要

It is critical to establish a direct and precise method with a high sensitivity and selectivity in analytical chemistry. In this research, making use of a well known phenomenon of capillary flow, we have proposed an image analysis method of nucleic acids at the price of a small amount of sample. When a droplet of the supramolecular complex solution, formed by neutral red and nucleic acids(NA) under an approximate neutral condition, was placed on the hydrophobic surface of dimethyl dichlorosilane pretreated glass slides, and it was evaporated, the supramolecular complex exhibited the periphery of the droplet due to the capillary effect, and accumulated there to form a red capillary-flow-directed assembly ring(CFDAR). A typical CFDAR has an outer diameter of (2r) about 1.18 mm and a ring width(2δ) of about 41 μm. Depending on the experimental conditions, a variety of CFDAR can be assembled. The experimental results are in agreement with our former theoretical discussion. It was found that when a droplet volume is 0.1 Μl, the fluorescence intensity of the CFDAR formed by the NR-NA is in proportion to the content of calf thymus DNA in the range of 0-0.28 ng, fish sperm DNA of 0-0.24 ng and yeast RNA of 0-0.16 ng with the limit of detection(3σ) of 1.7, 1.4 and 0.9 pg, respectively for the three nucleic acids.
机译:建立分析化学中具有高灵敏度和选择性的直接而精确的方法至关重要。在这项研究中,利用众所周知的毛细流动现象,我们提出了一种以少量样品为代价的核酸图像分析方法。将由中性红和核酸(NA)在近似中性条件下形成的超分子复合物溶液的液滴放在二甲基二氯硅烷预处理的载玻片的疏水表面上,并蒸发后,超分子复合物呈现出液滴由于毛细作用而聚集,并在那里聚集形成红色的毛细流导向装配环(CFDAR)。典型的CFDAR的外径(2r)约为1.18毫米,环宽(2δ)约为41μm。根据实验条件,可以组装各种CFDAR。实验结果与我们以前的理论讨论一致。发现当液滴体积为0.1μl时,由NR-NA形成的CFDAR的荧光强度与0-0.28ng范围内的小牛胸腺DNA的含量成正比,0-0.24的鱼精DNA ng和酵母RNA为0-0.16 ng,三种核酸的检出限(3σ)分别为1.7、1.4和0.9 pg。

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