VECTOR AND METHOD FOR EXPRESSION AND SELECTION randomized PEPTIDE SEQUENCES

• 1. 一种基质金属蛋白酶酶切序列肽、表达载体、多核苷酸序列及应用 [P] . 中国专利： CN105541969A . 2016-05-04
• 2. 降血压肽的串联多肽、表达载体、多核苷酸序列及应用 [P] . 中国专利： CN1911957A . 2007-02-14
• 3. the vector and a method for expression and selection of randomized peptide sequences [P] . ATA10372000A . 2002-07-15

  机译： 载体以及表达和选择随机肽序列的方法

• 4. collection selection method, methods for determining amino acid sequences and for producing a cvp or collection of nucleic acids, cvp, collections of vaccine peptides, nucleic acids, one or more expression vectors, and collection of vector vectors expression [P] . BR112020000590A2 . 2020-07-14

  机译： 收集选择方法，确定氨基酸序列和产生核酸的cvp或收集的方法，cvp，疫苗肽，核酸的收集，一种或多种表达载体以及表达载体的表达

• 5. Vector and a method for expression and selection of random peptide sequences [P] . 美国专利： US2004110281A1 . 2004-06-10

  机译： 载体以及表达和选择随机肽序列的方法

• 1. In Vivo Selection of Phage Sequences and Characterization of Peptide-specific Binding to Breast Cancer Cells [J] . Rui Wang, Ruifang Niu, Lin Zhang, 癌症生物学与医学（英文版 ） . 2008,第002期
• 2. Transferring a Gene Expression Cassette Lacking the Vector Backbone Sequences of the 1Ax1 High Molecular Weight Glutenin Subunit into Two Chinese Hexaploid Wheat Genotypes [J] . SHI Nong-nong, HE Guang-yuan, LI Ke-xiu, 农业科学学报（英文版） . 2007,第004期
• 3. Construction and Identification of Mammary Gland- specific Expression Vector of Bovine Tracheal Antimicrobial Peptide （TAP） [J] . Suizhong CAO, Xueping YAO, Yafei CUI, 农业生物技术：英文版 . 2013,第001期
• 4. A New Feature Selection Method for Improving the Precision of Diagnosing Abnormal Protein Sequences by Support Vector Machine and Vectorization Method [C] . Eun-Mi Kim, Jong-Cheol Jeong, Ho-Young Pae, Adaptive and Natural Computing Algorithms pt.2; Lecture Notes in Computer Science; 4432 . 2007
• 5. Central Limit Theorems for Randomly Modulated Sequences of Random Vectors with Resampling and Applications to Statistics [D] . Bagyan, Armine 2015
• 6. Selection of functional signal peptide cleavage sites from a library of random sequences. [O] . T Palzkill, Q Q Le, A Wong, 1994
• 7. Selection of v-Abl Tyrosine Kinase Substrate Sequences from Randomized Peptide and Cellular Proteomic Libraries Using mRNA Display [O] . Cujec Thomas P, Medeiros Patricia F, Hammond Phil, 2002

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Method of Designing Generators of Pseudorandom Sequences for Information Protection Based on Shift Register with Non-Linear Feedback Function [J] . Saleh Al-Omar . 信息安全（英文） . 2014,第4期
• 3. 抗菌肽Shiva-1a基因真核表达载体的构建及序列分析 [C] . 宫晓炜 ,郑福英 ,蔺国珍 . 第3届全国人畜共患病学术研讨会 . 2011
• 4. 牛消化道Ⅰ型肽载体(bPepT Ⅰ)部分序列的克隆、表达及多克隆抗体的制备 [A] . 赵秀新 . 2010