procedures for the specific coupling of a working party on the 5 '- end of mrna - fragmentes and the production of mrna and full cdna

摘要

PCT No. PCT/FR96/00651 Sec. 371 Date Oct. 31, 1997 Sec. 102(e) Date Oct. 31, 1997 PCT Filed Apr. 29, 1996 PCT Pub. No. WO96/34981 PCT Pub. Date Nov. 7, 1996The present invention relates to a method for the specific coupling of the cap of the 5' end of a eukaryotic mRNA fragment by a molecule functionalized by an amine functional group. The present invention also provides a method for isolating mRNA 5' end and a method for the preparation of single-stranded cDNA end complementary to the 5' end of mRNA and of double-stranded cDNA corresponding to the mRNA 5' end. The present invention also provides a method for isolating whole-length cDNA corresponding to the entire mRNA.