Incubate the cells on the surface of the support that is coated with fibrin until saturation, continue the culture for a sufficient amount of time to decompose the fibrin on the cell bottom, and then attach the cultured cells to the surface of the support A method for producing a cell sheet, comprising peeling from a sheet into a sheet. A support for preparing a cell sheet, the surface of which is coated with fibrin. A composition for coating the surface of a support for producing a cell sheet with fibrin, comprising fibrinogen and thrombin. A cell sheet can be produced by a simple operation using a support coated with a familiar material that is commercially available and has been confirmed to be safe.
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