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《世界糖尿病杂志:英文版(电子版)》
>Syndecan-1-coating of interleukin-17-producing natural killer T cells provides a specific method for their visualization and analysis
Syndecan-1-coating of interleukin-17-producing natural killer T cells provides a specific method for their visualization and analysis
Natural killer T cells(NKT cells) are innate-like T cells that acquire effector functions while developing in the thymus, polarize into three distinct functional subsets viz. NKT1, NKT2 and NKT17 cells that produce interferon(IFN)-γ, interleukin(IL)-4 and IL-17, respectively. However, there has been no unique surface markers that define each subsets, forcing investigators to use intracellular staining of transcription factors and cytokines in combination of surface markers to distinguish among these subsets. Intracellular staining, however, causes apoptosis and prevents subsequent utilization of NKT cells in functional in vitro and in vivo assays that require viable cells. This limitation has significantly impeded understanding the specific properties of each subset and their interactions with each other. Therefore, there has been fervent efforts to find a specific markers for each NKT cell subset. We have recently identified that syndecan-1(SDC-1; CD138) as a specific surface marker of NKT17 cells. This discovery now allows visualization of NKT17 in situ and study of their peripheral tissue distribution, characteristics of their TCR and viable sorting for in vitro and in vivo analysis. In addition, it lays the ground working for investigating significance of SDC-1 expression on this particular subset in regulating their roles in host defense and glucose metabolism.
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机译:A Primer of Fire Protection Measures and The Appropriate Selection of Flammability Test Methods for Materials and Products for Specific, End-Use Applications
机译:metodi microbiologici Tradizionali e metodi moleculeolari per l'analisi degli integratori alimentari a base di o con probiotici per ujso umano(microbiological and molecular methods for analysis of probiotic Based Food supplements for Human Consumption)。
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