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Methods and systems for high homologous recombination (“HR”) targeting efficiency

机译:高同源重组(“ HR”)靶向效率的方法和系统

摘要

Disclosed are vectors, kits and methods useful in the construction of recombinant cells and DNAs via enhanced efficiency homologous recombination. The vectors are targeting vectors that contain a gene-of-interest spliced between two ends that are homologous to a genome target site. The ends of the vector may be protected from exonuclease attack by deploying a cap, such as a hair pin structure. The vector is linked to a nuclear localization signal sequence, and preferably, a bait peptide that binds to RAD51, to facilitate homologous recombination. The vector may be deployed in myriad genetic transformation applications, such as site-directed mutagenesis, gene therapy, and the like.
机译:公开了用于通过增强的效率同源重组来构建重组细胞和DNA的载体,试剂盒和方法。载体是靶向载体,其包含在两个与基因组靶位点同源的末端之间剪接的目的基因。可以通过部署诸如发夹结构的帽来保护载体的末端免受核酸外切酶的攻击。载体与核定位信号序列连接,优选与与RAD51结合的诱饵肽连接,以促进同源重组。可以将载体部署在无数的遗传转化应用中,例如定点诱变,基因治疗等。

著录项

  • 公开/公告号US7892823B2

    专利类型

  • 公开/公告日2011-02-22

    原文格式PDF

  • 申请/专利权人 UTHAYASHANKER EZEKIEL;

    申请/专利号US20070702865

  • 发明设计人 UTHAYASHANKER EZEKIEL;

    申请日2007-02-06

  • 分类号C12N15/63;C12N15/11;

  • 国家 US

  • 入库时间 2022-08-21 18:08:58

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