METHOD FOR OBTAINING TRANSGENIC PLANTS EXPRESSING A PROTEIN WITH ACTIVITY PRODUCING HYDROGEN PEROXIDE BY TRANSFORMATION BY AGROBACTERIUM RHIZOGENES

摘要

The subject of the present invention is a method for obtaining transgenicplantsexpressing a protein with H2O2 producing activity, which comprises thefollowingsteps of:(a) transforming plant cells with Agrobacterium rhizogenes containing a vectorcarrying a gene encoding a protein producing H2O2 in a context which allowsits expression in the plant;(b) selecting the transformants which contain and express this gene, by aperoxidase-based colorimetric test;(c) regenerating the plants from the roots selected and monitoring theexpressionof the plantlets obtained by a peroxidase-based colorimetric test;(d) sorting according to phenotype and optionally carrying out the molecularanalysis of the progeny of the transgenic plants, allowing the selection ortheconfirmation of transgenic plants obtained containing only the transgene andnot the T-DNA specific to A. rhizogenes.