Soybean hairy roots produced in vitro by Agrobacterium rhizogenes-mediated transformation

摘要

Soybean is one of the world''''s most important oil and protein crops.Efficient transformation is a key factor for the improvement of soybean by genetic modification.We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro.Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants.After infection and co-cultivation,hairy roots were produced in induction culture medium after 10–12 days.Using this method,90%–99%of the infected explants of five different cultivars produced hairy roots within one month.Observations using reporter constructs showed that 30%–60%of the hairy roots induced were transformed.Based on high transformation efficiency and short transformation period,this method represents an efficient and rapid platform for study of soybean gene function.