One-step generation of composite soybean plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation

摘要

One-step ARM transformation of soybean. 7-day-old seedlings, ideal stage for transformation (a); The primary root was cut and the slant cut of hypocotyl (coated the bacterial mass) was scraped on the plate grown K599 (HGCI) (b); Close-up of section b marked in the box (c); Inoculated explants were planted into vermiculite and watered K599 (HGCI) (d); Inoculated seedlings were covered with a plastic bag (e); Induced hairy roots at 16 dpi (f). Scale bars = 1 cm