ACYLAMIDASE ENZYME, DNA FRAGMENT CODING SAID ENZYME, AND BIOCATALYTIC METHOD FOR SYNTHESIS OF N-SUBSTITUTED ACRYLAMIDES USING ACYLAMIDASE

摘要

FIELD: chemistry.;SUBSTANCE: invention discloses acylamidase enzyme AA37 from Rhodococcus erythropolis 37 All-Russian collection of industrial microorganisms Ac-1793, with a sequence given in the description. A nucleotide sequence which codes this enzyme is defined. The invention describes a method for synthesis of N-substituted acrylamides in aqueous medium from acrylamide and amines in the presence of an acylamidase biocatalyst in isolated state or in a composition with E.coli cells.;EFFECT: invention enables to obtain N-substituted aliphatic acrylamides from acrylamide and primary aliphatic amines in an aqueous medium.;3 cl, 14 ex