Isolated Nucleic Acid fragment which encodes Enzymes that regulate the biosynthesis of branched amonoacidos and use of Seeds and plants, ANOINT Chimeric, a host Cell transformedA polypeptide; a method for altering the level of expression of these Enzymes, Methods of obtaining

摘要

Reveal isolated Fragments of nucleic acids which encode enzymes involved in Plant biosu00e9ntesis of branched amino acids and their portions.These Enzymes are dihidroxiu00e1cido dehydratase, different isoenzymes of the branched chain amino acid aminotransferase,Of the 3 subunit Leuc leud isopropylmalate dehydratase and the 3 subunit of isopropylmalate dehydratase.It also describes the Chimeric genes containing Fragments of nucleic acids mentioned above and host cells transformed with these Chimeric genes.These polypeptides codificadospor Fragments of nucleic acids and Methods for allterar their expression level are also included.Said compositions are also described that they understand the polynucleotide and polypeptide.They also Reveal,Methods for obtaining vegetalesinvolucradas polynucleotides that encode enzymes in the biosynthesis of branched chain amino acids and finally a method to assess the ability of a CompoundTo inhibit the activity of a Plant Enzyme involved in the biosynthesis of branched chain amino acids.