METHOD FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES IN BLOOD USING FLUORESCENCE AND ABSORPTION OF A COLORANT

摘要

A kind of method for analyzing blood sample is provided, includes the following steps: a kind of blood sample) offer is with one or more first compositions and one or more secondary phases, and these secondary phases are different from the first component; Step b) deposited samples enter analysis room and are suitable for still sample being kept to be analyzed, and the chamber is by the first plate and the second plate, and wherein plate is transparent; Step c) mixed colorants and sample, these colorants are done so that first composition, and secondly to the component to fluoresce in the light for being exposed to scheduled first wave length, these light are performed the light for absorbing the predetermined second wave length of one or more of colorant; Step d) irradiates at least part of ingredient of sample and above-mentioned secondary phase initially includes first wave length and second wave length; The sample of step e) at least part imagings, the first composition and above-mentioned secondary phase of the original component of fluorescent emission with above-mentioned secondary phase and above-mentioned optical density are shown including generating picture signal; Step f) determines fluorescent value to first component, and secondly component uses the picture signal; The OD value of step g) determines each first composition, and secondly component, the function of these optical density (OD)s use picture signal by colorant absorbent components; H identifies the first composition and above-mentioned secondary phase, and uses identified fluorescence optical density value.