A method for analyzing a blood sample comprising the steps of: depositing a blood sample in an analysis chamber (10) adapted for resting the blood sample for analysis, the chamber (10) defined being by a first panel (12) and a second panel (16), both panels (12, 16) transparent, taking the blood sample one or more first constituents and one or more second constituents, being different second constituents of the first constituents; admixing a colorant with the blood sample being operative dye to cause the first constituents and the second constituents to fluoresce after exposure to a first wavelength of predetermined light, and being operative dye to absorb light in one or more second predetermined wavelengths of light; illuminating at least a part of the blood sample containing the first constituents and the second constituents selectively with the first wavelength and the second wavelength form; imaging at least a portion of the blood sample, including producing signals indicative image of fluorescent emissions from the first constituents and the second constituents and the optical density at the second wavelength of the first constituents and the second constituents; determining one or more fluorescent emission values for each the first constituents and the second constituents using the image signals; determining one or more optical density values at second wavelengths for each of the first constituents and the second constituents, the optical density being a function of the colorant absorbed by the constituents, using the image signals; and identifying the first constituents and the second constituents using the fluorescent emission values and optical density determined.
展开▼
