METHODS FOR ISOLATING ENDOGENOUS NUCLEIC ACIDS FROM SUBCELLULAR COMPARTMENTS WITHOUT FRACTIONATION

摘要

Methods of determining subcellular localization of nucleic acids, including RNA and DNA are described. In particular, the invention relates to a method combining proximity-specific labeling with crosslinking of nucleic acids to proteins and sequencing to identify nucleic acids within or near a particular subcellular compartment in vivo.