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BIOREACTOR FOR IN VITRO PLANT CULTURE

机译:体外植物培养生物反应器

摘要

The present utility model aims to provide a bioreactor for the in vitro cultivation of plants of the temporary immersion type comprising: (i) an upper container (2) with transparent walls for the material being propagated, preferably explants of eucalyptus, said container (2) being provided with (a) gas exchange diffuser to the external environment, (b) humidifier (12), and (c) artificial illumination (14); (ii) a lower container (3) with transparent walls for the liquid nutrient medium, having an aluminum tray (15) and a point (13) of water entry and/or nutrient medium, located in the bottom of said container (3); (iii) points (4) of injection/removal of air/oxygen/carbon dioxide located in the transparent lid (5); (iv) point (11) of additional injection of carbon dioxide with a drip line, close to the bottom of said upper container (2); (v) means (6) of hermetic connection between the upper and lower containers for supply/drainage of the nutrient medium to and from the upper container; (vi) a screen support (7) for the material to be propagated, with the possibility to exchange screens of different meshes; (vii) means (8) for locking the lid to hermetically close the upper container; (viii) means (9) for locking the lid to hermetically close the lower container; and (ix) means (10) for pneumatic drive of the liquid nutrient medium between said upper container and said lower container.
机译:本实用新型的目的是提供一种用于体外培养临时浸没型植物的生物反应器,该生物反应器包括:(i)上部容器(2),其具有用于繁殖的材料,优选为桉树外植体的透明壁,所述容器(2) )设有(a)与外界环境的气体交换扩散器,(b)加湿器(12),以及(c)人工照明(14); (ii)下部容器(3),其具有用于液体营养介质的透明壁,该下部容器(3)位于所述容器(3)的底部,具有铝制托盘(15)和进水和/或营养介质的尖端(13)。 ; (iii)位于透明盖(5)中的空气/氧气/二氧化碳的注入/排出点(4); (iv)在靠近所述上部容器(2)底部的地方用滴注线额外注入二氧化碳的点(11); (v)上部和下部容器之间的气密连接装置(6),用于将营养介质进出上部容器或从上部容器中排出; (vi)待传播物料的筛网支架(7),可以交换不同网眼的筛网; (vii)用于锁定盖以气密地关闭上部容器的装置(8); (viii)用于锁定盖子以气密地封闭下部容器的装置(9); (ix)用于在所述上部容器和所述下部容器之间气动驱动液态营养介质的装置(10)。

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