the present invention relates to genetic constructs containing two reporter genes such as rapporteur operon.one of the genes is linked to a peptide tag is recognized by specific proteases of trans translation.the invention also relates to the use of these genetic constructs, plasmids comprising a genetic construct.host and bacterial cells containing such a plasmid or a building to study the translational activity in vivo and trans for the identification of compounds capable of inhibiting the trans- translation and therefore likely to be of interest as an antibiotic.
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