Method of measuring creatine kinase MB isozyme and kit used therefor

摘要

The present invention addresses the problem of providing a method for specifically measuring CK-MB while avoiding an influence of CK-BB. That is, the present invention relates to "A method for measuring CK-MB in a sample, comprising: a step for reacting the sample with a first antibody against CK-MB, a second antibody against CK-MB which recognizes an epitope different from that recognized by the first antibody, and an anti-CK-B antibody which recognizes a region of the 1st to 100th amino acid from N-terminus of the amino acid sequence of the subunit B of creatine kinase (step 1); a step for measuring an optical change generated by the reaction (step 2); and a step for determining a quantity of CK-MB in the sample on the basis of the result obtained in the step 2 (step 3), and a kit to be used in the method, and a method for avoiding a influence of creatine kinase BB isozyme in measuring CK-MB, comprising treating a sample with the anti-CK-B antibody."