Method for measuring creatine kinase MB isozyme and kit used therefor

摘要

It is an object of the present invention to provide a method for specifically measuring CK-MB while avoiding the influence of CK-BB. That is, the present invention relates to a “method for measuring CK-MB in a sample comprising the following steps; a sample, a first antibody against CK-MB, and a second antibody against CK-MB having a different epitope from that of the first antibody; , A step of reacting with an anti-CK-B antibody that recognizes the 1st to 100th region from the N-terminal of the amino acid sequence of the B subunit of creatine kinase (step 1), and a step of measuring an optical change caused by the reaction (Step 2) Based on the results obtained in Step 2, the step of determining the amount of CK-MB in the sample (Step 3), the kit used therefor, and the sample with the anti-CK-B antibody CK-BB influence avoidance method in CK-MB measurement, characterized by processing.