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BACTERIAL STRAIN AND METHOD FOR HIGH THROUGHPUT OF SUGAR IN THE MICROBIAL CONVERSION INTO BIOSYNTHETIC PRODUCTS
BACTERIAL STRAIN AND METHOD FOR HIGH THROUGHPUT OF SUGAR IN THE MICROBIAL CONVERSION INTO BIOSYNTHETIC PRODUCTS
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机译:微生物转化为生物合成产物的高糖发酵细菌菌株及方法
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摘要
The present invention relates to recombinant Escherichia coli (E. coli) host cells comprising, in relation to wild-type cells, at least one mutation selected from the group consisting of deletion of the gene relA (ΔrelA); amino acid substitutions R290E and K292D in the protein guanosine-3′,5′-bis pyrophosphate 3′-pyrophosphohydrolase (bifunctional (p)ppGpp synthetase II; SpoT) (spo T[R290E;K292D]); and amino acid substitution G267C in the protein pyruvate dehydrogenase subunit E1 (AceE) (aceE[G267C]). Said recombinant host cells are characterized by increased sugar uptake rates that lead to increased productivity when using said cells for the production of biosynthetic products. The present invention further relates to respective methods for the biosynthetic production of a product of interest using said host cells.
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机译:重组大肠杆菌宿主细胞本发明涉及重组大肠杆菌宿主细胞,其相对于野生型细胞包含至少一种选自缺失的突变。基因relA的基因(ΔrelA);鸟苷3',5'-焦磷酸3'-焦磷酸水解酶(双功能(p)ppGpp合成酶II; SpoT)(spo T [R290E; K292D])中的氨基酸取代R290E和K292D;丙酮酸脱氢酶亚基E1(AceE)(aceE [G267C])中的氨基酸取代和G267C。所述重组宿主细胞的特征在于增加的糖摄取速率,当使用所述细胞生产生物合成产物时,其导致生产率提高。本发明进一步涉及使用所述宿主细胞生物合成生产目标产物的相应方法。
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