首页> 外国专利> Maltooligosyl Trehalose Trehalohydrolase (MTHase) Mutant and Application Thereof

Maltooligosyl Trehalose Trehalohydrolase (MTHase) Mutant and Application Thereof

机译：麦芽寡糖基海藻糖海藻水解酶（MTHase）突变体及其应用

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摘要

The present invention discloses a maltooligosyl trehalose trehalohydrolase (MTHase) mutant and application thereof, belonging to the technical fields of gene engineering and enzyme engineering. The present invention provides a series of MTHase mutants to prepare trehalose, having a better effect. Further, the MTHase mutant is expressed in Escherichia coli BL21 (DE3) and the enzyme is optimized by fermentation, which can significantly increase the yield of enzymes.

机译：本发明公开了一种麦芽寡糖基海藻糖海藻糖水解酶（MTHase）突变体及其应用，属于基因工程和酶工程技术领域。本发明提供了一系列制备更好的海藻糖的MTHase突变体。此外，MTHase突变体在大肠杆菌 BL21（DE3）中表达，并且该酶通过发酵进行了优化，可以显着提高酶的产量。 展开▼

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公开/公告号US2019249158A1

专利类型

公开/公告日2019-08-15

原文格式PDF

申请/专利权人 JIANGNAN UNIVERSITY;
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申请/专利号US201815991370

发明设计人 JING WU;LINGQIA SU;ZHENDONG WANG;
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申请日2018-05-29

分类号C12N9/24;C12P19/12;C12P19/14;

国家 US

入库时间 2022-08-21 12:10:53

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