The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
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机译：本发明涉及具有香叶基香叶基香叶基焦磷酸合酶活性的变体多肽，该变体多肽包含氨基酸序列，当与包含SEQ ID NO：1所示序列的香叶基香叶基焦糖基磷酸焦磷酸合酶比对时，其包含至少一个氨基酸取代对应于位置92、100或235的任何氨基酸的氨基酸残基，所述位置参考SEQ ID NO：1定义，并且其中所述变体与具有香叶基香叶基焦磷酸合酶活性的参考多肽相比具有一个或多个修饰的特性。本发明的变体多肽可以在重组宿主中用于生产甜菊醇或甜菊糖苷。
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