METHOD FOR PREPARING JM2 GENE KNOCKOUT MOUSE

摘要

Provided is a JM2 gene knockout mouse obtained by editing JM2 gene using a CRISPR-Cas9 system and by using somatic cell nuclear transfer techniques. Provided is a sgRNA designed for a CDS region of mouse JM2 gene. The gene is cleaved by using the CRISPR-Cas9 system to achieve the knockout of JM2 gene, and therefore a corresponding knockout mouse is obtained. The present invention provides a practicable method for mouse JM2 gene studies.