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Vibrating nanoneedle and lab-on-chip microfluidics system for single cell mechanics

机译:用于单细胞力学的振动纳米针和芯片实验室微流控系统

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摘要

Single cell mechanics is a vital part of single cell analysis. It has attracted great interest among scientists as cell mechanics can be linked to early diagnosis of diseases. To date, several great findings have been achieved in the study of single cell mechanics. Nevertheless, more work are required to enable the technology to be pushed to the frontier of single cell mechanics. Considering this objective, this work focuses on the technological development of two major parameters of single cell mechanics: Single Cell Wall (SCW) cutting operations (Phase 01) and Single Cell Mass (SCM) measurement (Phase 02). A saccharomyces cerevisiae yeast cell was used as a sample cell. In phase 01, a vibrating nanoneedle (tungsten) integrated with lead zirconate titanate piezoelectric actuator was used for SCW cutting operation. Two different frequencies of vibrating nanoneedle were used for cell wall cutting operation: 1 Hz and 10 Hz. For a constant penetration depth of 1.2 µm, the obtained cell nanoneedle’s velocities were 7 µm/s and 24 µm/s. Results show that faster nanoneedle causes less damage to the cell surface. In phase 02, a Lab-On-Chip microfluidics system was used for SCM measurement. SCM result was extracted from the relation between drag force applied on cell and Newton’s law of motion. Drag force on the cell has been generated by a pressure driven syringe micropump. This approach of measuring SCM was calibrated using a known mass (73.5 pico gram) of polystyrene particle of 5.2 µm diameter. Different sizes (2-7 µm diameter) of yeast cells were cultured in our laboratory. Mass of 4.4 µm diameter of yeast cell was measured as 2.12 pg. In addition, results show that single yeast cell mass increases exponentially with the increase of cell size. It is envisaged that this work i.e. combination of single cell cutting operation and single cell mass measurement system will add a significant contribution to the knowledge of cell mechanics and single cell analysis.
机译:单细胞力学是单细胞分析的重要组成部分。由于细胞力学可以与疾病的早期诊断联系起来,因此引起了科学家的极大兴趣。迄今为止,在单细胞力学研究中已经取得了一些重大发现。然而,需要更多的工作来使该技术被推向单细胞力学的前沿。考虑到这个目标,这项工作着重于单细胞力学的两个主要参数的技术发展:单细胞壁(SCW)切割操作(阶段01)和单细胞质量(SCM)测量(阶段02)。酿酒酵母酵母细胞用作样品细胞。在阶段01中,将集成有锆钛酸铅钛酸盐压电致动器的振动纳米针(钨)用于SCW切割操作。两种不同频率的振动纳米针用于细胞壁切割操作:1 Hz和10 Hz。对于1.2微米的恒定穿透深度,获得的细胞纳米针的速度分别为7微米/秒和24微米/秒。结果表明,更快的纳米针对细胞表面的损害较小。在阶段02中,将芯片实验室微流控系统用于SCM测量。 SCM结果是从施加在细胞上的阻力与牛顿运动定律之间的关系中提取的。压力驱动的注射器微型泵已产生对电池的拖曳力。这种测量SCM的方法是使用已知质量的直径为5.2 µm的聚苯乙烯颗粒(73.5皮克)进行校准的。在我们的实验室中培养了不同大小(直径2-7 µm)的酵母细胞。测得的酵母细胞直径为4.4 µm,质量为2.12 pg。另外,结果表明,单个酵母细胞的质量随细胞大小的增加而呈指数增加。可以设想,这项工作,即单细胞切割操作和单细胞质量测量系统的结合,将对细胞力学和单细胞分析的知识做出重大贡献。

著录项

  • 作者

    Rahman Md. Habibur;

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  • 年度 2015
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  • 原文格式 PDF
  • 正文语种 en
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