首页> 外文OA文献 >Multispacer Typing of Rickettsia prowazekii Enabling Epidemiological Studies of Epidemic Typhus†
【2h】

Multispacer Typing of Rickettsia prowazekii Enabling Epidemiological Studies of Epidemic Typhus†

机译:立克次体立克次体的多间隔分型使流行性斑疹伤寒的流行病学研究†

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Currently, there is no tool for typing Rickettsia prowazekii, the causative agent of epidemic typhus, currently considered a potential bioterrorism agent, at the strain level. To test if the multispacer typing (MST) method could differentiate strains of R. prowazekii, we amplified and sequenced the 25 most variable intergenic spacers between the R. prowazekii and R. conorii genomes in five strains and 10 body louse amplicons of R. prowazekii from various geographic origins. Two intergenic spacers, i.e., rpmE/tRNAfMet and serS/virB4, were variable among tested R. prowazekii isolates and allowed identification of three and two genotypes, respectively. When the genotypes obtained from the two spacers were combined, we identified four different genotypes. MST demonstrated that several R. prowazekii strains circulated in human body lice during an outbreak of epidemic typhus in Burundi. This may help to discriminate between natural and intentional outbreaks. Our study supports the usefulness of MST as a versatile method for rickettsial strain genotyping.
机译:目前,尚无用于在菌株水平上对流行性斑疹伤寒的病原体立克次氏体进行分类的工具,目前被认为是潜在的生物恐怖分子。为了测试多间隔分型(MST)方法是否可以区分普氏疟原虫的菌株,我们在5个菌株和10个普氏疟原虫的体虱扩增子之间扩增了R. prowazekii和R. conorii基因组之间的25个可变性最高的基因间隔子并进行了测序。来自不同的地理来源。两个基因间隔子,即rpmE / tRNAfMet和serS / virB4,在被测试的R. prowazekii分离株之间是可变的,并分别鉴定了三种和两种基因型。当将从两个间隔区获得的基因型合并时,我们确定了四种不同的基因型。 MST证明,在布隆迪爆发流行性斑疹伤寒期间,几种虱子R. prowazekii菌株在人体虱子中传播。这可能有助于区分自然爆发和故意爆发。我们的研究支持MST作为立克次体菌株基因分型的通用方法的有用性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号