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An in vivo Assay for Simultaneous Monitoring of Neuronal Activity and Behavioral Output in the Stomatogastric Nervous System of Decapod Crustaceans

机译:同时监测十足纲甲壳动物的胃胃神经系统中神经元活性和行为输出的体内分析。

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摘要

Central pattern generators (CPGs) generate rhythmic output patterns and drive vital behaviors such as breathing, swallowing, locomotion and chewing 1-10. While most insights into the rhythm generating mechanisms of CPGs have been derived from isolated nervous system preparations, the relationship between neural activity and corresponding behavioral expression is often unclear. The stomatogastric system of decapod crustaceans is one of the best characterized neural system for motor pattern generation 9-12 and many mechanisms of motor pattern generation and selection have been discovered in this system. Since most studies are limited to the isolated nervous system, little is known about the actual behavioral output of this system. For example, it is unknown whether the observed flexibility in the motor patterns is present in vivo and whether distinct motor activities drive corresponding behavioral patterns. We present a method which allows electrophysiological recordings of CPG neurons and the simultaneous monitoring of the behavioral output of the stomatogastric nervous system. For this, we use extracellular hook electrodes either for recording or stimulation of neurons in the gastric mill CPG that drive the chewing movements of three teeth in the foregut of the animal. Electrodes are applied in tethered, but otherwise fully intact crabs (Cancer pagurus) and an endoscope is used to monitor tooth movements. Nerve and video recordings of the endoscopic camera are synchronized and motion tracking techniques are used to analyze gastric mill movements. This approach thus allows testing the behavioral relevance of the neural activity patterns produced by central pattern generators.
机译:中央模式发生器(CPG)产生有节奏的输出模式,并驱动诸如呼吸,吞咽,运动和咀嚼等重要行为1-10。虽然大多数有关CPG的节律产生机制的见解都来自孤立的神经系统准备,但神经活动与相应行为表达之间的关系通常不清楚。十足类甲壳动物的胃胃系统是最能表征运动模式生成的神经系统之一,9-12,并且在该系统中发现了许多运动模式生成和选择的机制。由于大多数研究仅限于孤立的神经系统,因此对该系统的实际行为输出知之甚少。例如,尚不清楚体内是否存在观察到的运动模式灵活性,以及​​不同的运动活动是否驱动相应的行为模式。我们提出了一种方法,可以对CPG神经元进行电生理记录,并同时监视胃胃神经系统的行为输出。为此,我们使用细胞外钩形电极在胃磨CPG中记录或刺激神经元,这些神经元驱动动物前肠中三颗牙齿的咀嚼运动。电极用于拴系的螃蟹,但完全完好无损的螃蟹(巨蟹座)中使用内窥镜监测牙齿的运动。内窥镜摄像机的神经和视频记录是同步的,并且使用运动跟踪技术来分析胃磨的运动。因此,该方法允许测试由中央模式发生器产生的神经活动模式的行为相关性。

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