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Development of a lab-on-chip platform integrating electrochemical microsensors for the detection of water contaminants based on algal physiology monitoring

机译:集成电化学微传感器的芯片实验室平台的开发,用于基于藻类生理监测来检测水污染物

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摘要

The monitoring of water quality has been of great importance in recent decades in order to find solutions to control water contamination, largely driven by agricultural and industrial activities. Although conventional methods such as chromatography, are very accurate and sensitive tools, increasing interest has been placed on promising techniques that can be used on site, are low cost, and offer the ability to perform rapid analysis. The present work is devoted to the development of lab on a chip component to the analysis of the toxicity of water. It consists of a portable system for detecting on-site and offers the possibility of a double complementary detection: optical and electrochemical. As the part dedicated to the electrochemical sensor has been previously validated, this study focused on the implementation of an electrochemical biosensor based on the use of an alga, for the detection of pollutants in water. The detection basic principle consists in monitoring changes in the metabolic activity of algae induced by the presence of herbicides. The seaweed response is different for each concentration of herbicide in an examined sample. Two herbicides selected affect the photosynthetic activity of the algae and consequently, induce changes in the amount of electro-active species produced by the alga: O2, H2O2 and H3O + / OH-. Before the development of the final component type Lab on Chip, detection principles as well as the electrode materials that will be integrated, have been validated using a simpler type of component, which was achieved through the silicon manufacturing technologies and which was characterized by simpler procedures.A silicon chip containing an electrochemical micro incorporating three electrodes was established. Once validated detecting materials and previously selected settings were used in the manufacture of components on Lab Chip. The Lab-on-Chip components were then used for the biological tests to detect the herbicides of interest. Special attention has been placed on monitoring like O2 indicator of the presence of herbicide, because this element is the most representative of changes of metabolic activity. An inhibiting effect on photosynthesis, concentration dependent of the herbicide been demonstrated. Detection of herbicidal was performed with great sensitivity and a range of covering the boundary concentration Maximum acceptable taxed by the Canadian Government.
机译:在最近几十年中,水质监测一直非常重要,以便找到控制水污染的解决方案,而这主要是由农业和工业活动推动的。尽管常规方法(例如色谱法)是非常准确和灵敏的工具,但人们越来越关注可以在现场使用,价格低廉且具有执行快速分析功能的有前途的技术。目前的工作致力于在芯片部件上实验室的发展,以分析水的毒性。它由一个用于现场检测的便携式系统组成,并提供了双重互补检测的可能性:光学和电化学。由于先前已经验证了专用于电化学传感器的部件,因此本研究着重于基于藻类使用的电化学生物传感器的实施,以检测水中的污染物。检测的基本原理在于监测除草剂的存在引起的藻类代谢活性的变化。对于所检查样品中每种除草剂浓度,海藻响应均不同。选择的两种除草剂会影响藻类的光合活性,因此会诱导藻类产生的电活性物质的量发生变化:O2,H2O2和H3O + / OH-。在开发最终组件类型的芯片实验室之前,已经使用一种更简单的组件类型(通过硅制造技术实现了该组件的特点)进行了验证,并验证了检测原理以及将要集成的电极材料。建立了包含结合有三个电极的电化学微芯片的硅芯片。经过验证的检测材料和先前选择的设置将用于Lab Chip上组件的制造。然后将芯片实验室组件用于生物学测试,以检测目标除草剂。像O2指示剂一样,除草剂的存在已引起特别关注,因为该元素是代谢活性变化的最代表。证明了对光合作用的抑制作用,浓度取决于除草剂。除草剂的检测具有很高的灵敏度,检测范围涵盖了加拿大政府所接受的最大极限浓度。

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    Tsopela Aliki;

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  • 年度 2015
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  • 原文格式 PDF
  • 正文语种 en
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