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Type II Na+-Pi cotransporters in osteoblast mineral formation: Regulation by inorganic phosphate

机译:成骨细胞矿物质形成中的II型Na + -Pi共转运蛋白:无机磷酸盐的调控

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摘要

During calcification of bone, large amounts of phosphate (P(i)) must be transported from the circulation to the osteoid. Likely candidates for osteoblast P(i) transport are the type II sodium-phosphate cotransporters NaPi-IIa and NaPi-IIb that facilitate transcellular P(i) flux in kidney and intestine, respectively. We have therefore determined the 'cotransporters' expression in osteoblast-like cells. We have also studied the 'cotransporters' regulation by P(i) and during mineralization in vitro. Phosphate uptake and cotransporter protein expression was investigated at early, late and mineralizing culture stages of mouse (MC3T3-E1) and rat (UMR-106) osteoblast-like cells. Both NaPi-IIa and NaPi-IIb were expressed by both osteoblast-like cell lines. NaPi-IIa was upregulated in both cell lines one week after confluency. After 7 days in 3mM P(i) NaPi-IIa was strongly upregulated in both cell lines. NaPi-IIb expression was unaffected by both culture stage and P(i) supplementation. The expression of both cotransporters was unaffected by P(i) deprivation. In vitro mineralization at 1.5mM P(i) was preceded by a three-fold increase in osteoblast sodium-dependent P(i) uptake and a corresponding upregulation of both NaPi-IIa and NaPi-IIb. Their expression thus seem regulated by phosphate in a manner consistent with their playing a role in transcellular P(i) flux during mineralization. Copyright 2007 S. Karger AG, Basel.
机译:在骨骼钙化过程中,必须将大量磷酸盐(P(i))从循环系统运输到类骨质。成骨细胞P(i)转运的可能候选物是II型磷酸钠共转运蛋白NaPi-IIa和NaPi-IIb,它们分别促进肾脏和肠中跨细胞P(i)的通量。因此,我们确定了成骨细胞样细胞中的“共转运蛋白”表达。我们还研究了P(i)和体外矿化过程中的“共转运蛋白”调控。在小鼠(MC3T3-E1)和大鼠(UMR-106)成骨细胞样细胞的早期,晚期和矿化培养阶段研究了磷酸盐的吸收和共转运蛋白的表达。 NaPi-IIa和NaPi-IIb均由成骨细胞样细胞系表达。融合后一周,NaPi-IIa在两种细胞系中均上调。在3mM P(i)中培养7天后,NaPi-IIa在两种细胞系中均强烈上调。 NaPi-IIb表达不受培养阶段和P(i)补充的影响。两个共转运蛋白的表达不受P(i)剥夺的影响。在1.5mM P(i)体外矿化之前,成骨细胞钠依赖性P(i)摄取增加了三倍,并且NaPi-IIa和NaPi-IIb都相应上调。因此,它们的表达似乎受到磷酸盐的调节,与它们在矿化过程中在跨细胞P(i)通量中的作用一致。版权所有2007 S. Karger AG,巴塞尔。

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